⊙Principle : Inhibition of catechol oxidase activity by PTU -Inhibition : Enzyme inhibitors are molecules that interact in some way with the enzyme to prevent it from working in the normal manner. There is a variety of types of inhibitors including competitive and noncompetitive inhibition. -Competitive inhibition : A competitive inhibition is compound which closely resembles the chemical structure and molecular geometry of the substrate. The inhibitor competes for the same active site as the substrate molecule. The inhibitor may interact with the enzyme at the active site, but no reaction takes place. The inhibitor is stuck on the enzyme and prevents ant substrate molecules from reacting with the enzyme. However, a competitive reaction is usually reversible if sufficient substrate molecules are available to ultimately displace the inhibitor. Therefore, the amount of enzyme inhibition depends upon the inhibitor concentration, substrate concentration, and the relative affinities of the inhibitor and substrate for the active site.
⊙PrincipleCatechol oxidase is an enzyme that catalyses the oxidation of catechol. Catechol oxidase carries out the oxidation of catechol by using oxygen. In the presence of catechol and oxygen, benzoquinone is formed. Hydrogen are removed from catechol with oxygen to form water. This reaction is a form of enzymatic browning exhibited in many food like apples of bananas. <중 략>-Explain your results in terms of your hypothesis.I thought that if I combine catechol oxidase in potato extract and catechol, oxygen, benzoquinone will be made, in other words browning reaction will occur. Through this experiment , I knew that the reaction doesn`t occur in absence one of these. Also, the reaction occurs in presence of all. Therefore, my hypothesis was proved of true in this experiment