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[PPT] Hydration of Isobutylene to Tert-butanol

Batch Reactor 환경Langmuir-Hinshelwood-Hougen-Watson kinetic(LHHW) 파라미터입력은Subroutine없이는Reactive Distillation에적용불능Aspen Plus에LHHW 입력이가능한CSTR Reactor에적용하여Equilibrium으로근사한반응파라미터와비교시도Reactive Distillation은반응/분리가동시에진행됨으로주어진equilibrium 상수로반응속도구현<중 략>설계안전성기존Batch Reactor, CSTR대형누출위험분리공정단계추가로공정risk증가사고발생시누출유량조절어려움

공학/기술| 2015.10.10| 26페이지| 5,000원| 조회(145)

PPT, 발표, 공정설계, 창의설계, 경시대회, aspen, pro 2

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카이스트 분자공학실험 Adsorption(흡착) 결과 보고서

2013 Fall SemesterResult ReportChap. 1. Equilibrium : AdsorptionReporting Date : 2013. 11. 25Group No. : 3Student No. : 20112040Name : 冠荐泅1. Data Sheet of Calculation Results* The 6th datum was off the point of tendency so I excluded that datum point.2. Result and Discussion1) Data Plot based on Langmuir Theory2) Linear Plot based on 1)3) Data Plot based on BET Theory4) Linear Plot based on 3)5) Table of Surface Area6) Adsorption Isotherm (Wt versus Pe/P0)CommentsExplain why pretreatment processes are always necessary before a sample analysis. Next, consider why the weight of a sample should be measured after pretreatment.- Preserves the media for intended purpose.- Prevent premature exhaustion of media.- Reduces affect of interferents that can blind adsorption sites or foul media.- Prevents blocking of pore based adsorption sites where adsorption takes place.- To reduce the error because of particles around laboratory, make the sample in pure condition.Draw a comparison between Langmuir and BET according to your results.- Theorically Langmuir isotherm is about monolayer adsorption. So when using Langmuir isotherm the data should be more precise to the real value. Because there were some kinds of error maybe, we got the langmuir isother data bigger than BET data.What is the general purpose of adsorption experiment? Find one actual application of this experiment.- The goal of adsorption expeiment is to extract specific gas from the mixture of gases. The actual application of this experiment is to get high purity oxygen gases and to reduce nitrogen oxide or ammonia nitrogen.Reference1. CBE 201 Molecular Engineering Laboratoty, KAIST, 20132. Levine, Ira N. (2009) “"Physical Chemistry,”" 6th Ed. McGraw-Hill, Boston.3. Moore, Walter J. (1972). “"Physical Chemistry,”" 4th Ed. Prentice-Hall, New Jersey.4. Sime, Rodney J. (1990). “Physical Chemistry: Methods, Techniques and Experiments.” Saunders College Publishing, Philadelphia.5. Duff, David G., Ross, Sheina M.C. and Vaughan, D. Huw “Adsorption form Solution: AnExperiment to Illustrate the Langmuir Isotherm” J. Chem. Ed. 65 815 (1988).6. Atkins, P.W. Physical Chemistry 6th edition. San Francisco: W. H. Free-man and Company, 1998. 7. Derivation of the Langmuir and BET Isotherms8. Surface Adsorption

공학/기술| 2015.10.10| 7페이지| 2,000원| 조회(119)

보고서, 흡착, Adsorption, 결과, 분자공학실험

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홍익대학교 화학공학과 화공전산 과제 전체 모음 (오교수님꺼)

홍익대학교 화학공학과 화공전산 과제 전체 모음입니다. (오교수님꺼)Text book : Applied Numerical Methods with MATLAB 한학기 과제 이걸로 참고하면 끝!!

프로그램소스| 2015.10.10| 5,000원| 조회(326)

MatLab, 홍익대학교, 화학공학과, 화공전산

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카이스트 분자공학실험 Introduction of chemical and biomolecular engineering 결과 보고서

CBE 201 Final reportIntroduction to chemical and biomolecular engineeringGruop No. : 3Student ID : 20112040Name : Soohyun Park1. Experimental procedure① Choose a single colony from a freshly streaked bacterial plate and use it to inoculate LB plus an appropriate antibiotic. Incubate the culture overnight while shaking.② Harvest 3-5ml of bacterial culture by centrifugation at 13,000 rpm for 30 sec at RT and discard the supernatant.③ Resuspend the pellet in 250μl of resuspension buffer, vortexing or pipetting until no clumps of the cell pellet remain.④ Add 250μl of Lysis buffer to the resuspended cells. Close tube and gently mix by inverting the tube several times. Do not vortex and do not exceed 5 min of lysis time.⑤ Add 350μl of neutralization buffer and gently mix by inverting the tube several times.⑥ Centrifuge at 13,000 rpm for 10 min at 4℃. While waiting for the centrifugation, insert a column into the collection tube.⑦ After centrifugation, transfer supernatant promptly into the cre out that the target plasmid DNA were extracted as we designed.Figure 1. Plasmid Extraction KitFigure 2. DNA electrophoresis device2. Results and discussionResultsFigure3. Agarose gel electrophoresis of the plasmid1. Data analysis- The left side is DNA size marker.- The bio-marker shows the relative position of the E.coli.- Using ethidium bromide to dye DNA, if there is.- We could get the very good data from the experiment. First, it means that there is DNA. Second, it shows that all of the data are at the same position on the agarose gel which represent the data are at the plasmid DNA position.2. Caution- We should follow the exact protocol for extracting the plasmid DNA in E. coli .- For instance, pH is very important parameter for this alkaline denaturation method.- Make high concentration solution. Because if we make low concentration solution, we should conduct the procedure again to make high concentration solution. But if we have high concentration solution, we can make low coe conformations : supercoiled, open-circular, and linear. Plasmid DNA is tightly supercoiled circle to enable it to fit inside the cell. Following a careful plasmid prep, most of the DNA will remain supercoiled, but a certain amount will sustain single-strand nicks. Given the presence of a break in only one of the strands, the DNA will remain circular, but the break will permit rotation around the phosphodiester backbone and the supercoils will be released. A small, compact supercoiled knot of DNA sustains less friction against the agarose matrix than does a large, floppy open circle. Therefore, for the same DNA. A smaller over-all size, supercoiled DNA runs faster than opencircular fraction of the DNA sustains double-strand breaks, producing a linear conformation. Linear DNA runs through a gel end first and thus sustains less friction than open-circular DNA, but more than supercoiled. So 7 Lane is 'Open circular', 8 Lane is 'linear', and 9 Lane is 'supercoiled'. And we will consider awere purchased from Invitrogen (Carlsbad, CA). pVAX1-GFP (3642 bp) was constructed by cloning the superfolding green fluorescent protein (sGFP) gene into the multi-cloning site of pVAX1. The sGFP gene was obtained from pTrcsGFP, a gift from the Gregory Stephanopoulos laboratory (Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA). pCP40 (5029 bp) was constructed by Remaut et al. and was obtained from the Belgian Coordinated Collections of Microorganisms BCCM/LMBP plasmid collection (accession number LMBP 951). To construct pDMB02-GFP, a 1938-bp fragment of pVAX1 containing the human cytomegalovirus (CMV) immediate-early promoter/enhancer, bovine growth hormone (BGH) polyadenylation signal, and kanamycin resistance gene was PCR-amplified using primers containing AvrII and SbfI restriction sites. A 3073-bp fragment of pCP40 containing the R1 origin of replication along with the repA, copA, and copB gene sequences was also PCRamplified with the s:5’-GTGAAGATCAGTCATACCATCCTGCACTTACAATGCG-3’5’-GCAGGATGGTATGACTGATCTTCACCAAACGTATTACCG-3’After PCR, the template plasmid was digested using DpnI, and after clean-up the reaction was used to transform ElectroMAX DH10B cells (Invitrogen). Positive transformants were selected on LB/agar plates containing 50 μg/mL kanamycin, and the presence of the start codon mutation was verified by sequencing.In this work, we have constructed a new DNA vaccine vector, pDMB02-GFP, containing the runaway R1 origin of replication. The runaway replication phenotype should result in plasmid copy number amplification after a temperature shift from 30°C to 42°C. However, using Escherichia coli DH5α as a host, we observed that the highest yields of pDMB02-GFP were achieved during constant-temperature culture at 30°C, with a maximum yield of approximately 19 mg pDNA/g DCW being observed. By measuring mRNA and protein levels of the R1 replication initiator protein, RepA, we determined that RepA may be limiting pD3763

공학/기술| 2015.10.10| 4페이지| 2,000원| 조회(124)

보고서, 카이스트, 결과, mini prep, 분자공학실험

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카이스트 분자공학실험 The optical properties of organic photoeletric materials 결과 보고서

CBE 201 Final reportThe optical properties of organic photoelectric materialsGruop No. : 3Student ID : 20112040Name : Soohyun Park1. Experment procedureA. Preperation of organic thin-film① Prepare the glasses size in 1.5 X 1.5 cm. The glass was prepared by assistance for rapid procedure of our experiment.② Put the glasses into plasma ashing system to make glass familiar with the solution.③ Set the spin-coater to 25 seconds for 700RPM, 2 seconds for lamp time. And then put the glasses on the spin-coater with polymer solution by using the spuit. The color of the polymer solution turn into purple from orange Which means it turned into solid state.④ After coat the glasses, make three different types of samples. Make six samples and each type of the samples are two pieces respectively. For the first two samples put on the hot plate for annealing. and other samples make as it is. and the other samples are pure glasses.⑤ Prepare the solution with 1ml cuvette for measure the absorption differe53nm.- As we know the equation E = hc/λ = (1240 eV · nm)/λ(nm). we can convert to electron volts.FilmAnnealed filmSolutionE(eV) max.2.2542.2922.737Table 1. Electron volts of each sample- According to the date analysis, we could compare the E between three samples. Short wavelength shows more effective than long wavelength. It represent annealed film is better than non-annealed film.2) Photo counter meterFigure 4. P3HT_PL- Analyzing Fig 1. and Fig 4. we could conclude that the energy decreases as wavelength of light increase, absorbed energy is bigger than emitted energy. The reason of this matter can be explained by the phenomena that molecules consume part of absorbed energy into their own vibrational energy in the process of absorption and emission of light.A. Let's understand the mechanism of light absorption as well as that of the photoluminescence spectrum.- The formalism for the absorption of light in the simplest case starts with a beam of light of intensity I of photons that hable bond added, the system absorbs photons of longer wavelength (and lower energy), and the compound ranges from yellow to red in color. Compounds that are blue or green typically do not rely on conjugated double bonds alone. This absorption of light in the ultraviolet to visible spectrum can be quantified using ultraviolet visible spectroscopy, and forms the basis for the entire field of photochemistry. Conjugated systems that are widely used for synthetic pigments and dyes are diazo and azo compounds and phthalocyanine compounds.Measurement of light absorption and photoluminescence spectrum is conducted by UV-Vis spectrophotometer and photo counter meter respectively. In UV-Vis spectrophotometer, light is radiated first by source such as tungsten filament or a deuterium arc lamp. Then, radiated light is divided into two beams through splitter before it reaches the sample. One beam is used as the reference and the other beam is made to pass through the sample. Detectors measure the in of peak is broad, so we can expect that the band gap is not quite broad.C. What is the difference in photoluminescence between the liquid state and solid state? Why?- In a liquid state, solution, the formation of organic solar cell polymer is randomly coiled. However, in solid state, organic solar cell polymer shows better packed π-π layers. Better and organized π-π layers finally affect to increase the power conversion efficiency. Because of this, there is a difference in photoluminescence between a liquid state and a solid state. Photoluminescence has fluorescence and phosphorescence. Commonly, fluorescence frequently occur in solution, so brightness is the same or higher at low temperature. And, phosphorescence frequently occur in solid, so brightness is lower at low temperature. Therefore, as only consideration for the fluorescence, organic fluorescence materials show good efficiency of fluorescence at solution, but they show not good efficiency of fluorescence at solid because th but there are few effects on the brightness of fluorescence. When the humidity is increased, the brightness is decreased because of extinction.- Oxygen: When the oxygen molecules exist, extinction occur, so the intensity of fluorescence is decreased. The reasons are that the material become oxidation, and the paramagnetism of the oxygen molecules.F. [Extra problem #1] 실험과정 중 O2 plasma 를 사용하였는데 그 이유를 설명하시오.- Oxygen plasmas have been widely used in many processes. Improving adhesion between two surfaces is a common application. Good adhesion requires strong interfacial forces via chemical compatibility and/ or chemical bonding. Plasma surface treatment can also assist in creating chemically active functional groups, such as amine, carbonyl, hydroxyl and carboxyl groups, to improve interfacial adhesion. Using oxygen Plasma generate reactive species to improve bondability on substrates such as glass.G. [Extra problem #2] Annealed P3HT film의 PL spectrum과 P3HT:PCBM film의 PL spectrum을 그려보아 비ny

공학/기술| 2015.10.10| 5페이지| 2,000원| 조회(132)

보고서, 유기, 카이스트, optical, 결과, 광전소자, 분자공학실험

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