Simultaneous determination of baicalin and glycyrrhizin in Sosiho-tang by HPLC and anti-inflammatory activity

서지정보

· 발행기관 : 대한본초학회
· 수록지 정보 : 대한본초학회지 / 26권 / 1호 / 41 ~ 46페이지
· 저자명 : 김정훈, 신현규, 하혜경, 서창섭, 이진아

초록

Objectives：To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated.
Methods：A quantitative analysis was performed using a high performance liquid chromatography (HPLC).
Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO)and prostaglandin (PG)E2 were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats.
The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats.
Results：The correlation coefficients of the compounds showed good linearity (r^2>0.9992) over the linear range.
The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41–101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on PGE2 production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats.
Conclusions：The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.

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