HPLC를 이용한 정공등의 다성분 동시함량분석 (Simultaneous Quantification Analysis of Multi-components on Erycibae Caulis by HPLC)

서지정보

· 발행기관 : 대한약학회
· 수록지 정보 : 약 학 회 지 / 57권 / 4호 / 272 ~ 281페이지
· 저자명 : 전혜진, 유정, 황완균

초록

In this study, we developed and validated the HPLC method using the isolated components from Erycibae caulis.
Their structures were elucidated by spectroscopic methods including UV, 1H-NMR, 13C-NMR, FAB-Mass and ESI-Massas Compound 1 (crypto-chlorogenic acid), Compound 2 (scopolin), Compound 3 (neochlorogenic acid) and Compound 4 (3,4-di-O-caffeoylquinic acid). Major three compounds and scopoletin were decided as representative components of Erycibaecaulis. We established HPLC analytical method by using the representative components and 20 commercial samples whichwere collected considering to various cultivated area. The HPLC fingerprinting was successfully achieved with an AKZONOBEL Kromasil 100-5C18 column. The mobile phase consisted of 0.5% acetic acid in water (A) and methanol (B) usinggradient method of 85(A) to 50(A) for 35min. The fingerprints of chromatograms were recorded at an optimized wavelengthof 330 nm. This developed analytical method was validated with specificity, selectivity, accuracy and precision. And it is suggestedthat scopolin, scopoletin, neochlorogenic acid, 3,4-di-O-caffeoylquinic acid were more than 0.162%, 0.133%, 0.057%,0.044%, respectively. In addition, principal component analysis (PCA) was performed on the analytical data of 20 differentErycibae caulis samples in order to classify samples collected from different regions. We hope that this assay can be readilyutilized as quality control method for Erycibae caulis.

영어초록

In this study, we developed and validated the HPLC method using the isolated components from Erycibae caulis.
Their structures were elucidated by spectroscopic methods including UV, 1H-NMR, 13C-NMR, FAB-Mass and ESI-Massas Compound 1 (crypto-chlorogenic acid), Compound 2 (scopolin), Compound 3 (neochlorogenic acid) and Compound 4 (3,4-di-O-caffeoylquinic acid). Major three compounds and scopoletin were decided as representative components of Erycibaecaulis. We established HPLC analytical method by using the representative components and 20 commercial samples whichwere collected considering to various cultivated area. The HPLC fingerprinting was successfully achieved with an AKZONOBEL Kromasil 100-5C18 column. The mobile phase consisted of 0.5% acetic acid in water (A) and methanol (B) usinggradient method of 85(A) to 50(A) for 35min. The fingerprints of chromatograms were recorded at an optimized wavelengthof 330 nm. This developed analytical method was validated with specificity, selectivity, accuracy and precision. And it is suggestedthat scopolin, scopoletin, neochlorogenic acid, 3,4-di-O-caffeoylquinic acid were more than 0.162%, 0.133%, 0.057%,0.044%, respectively. In addition, principal component analysis (PCA) was performed on the analytical data of 20 differentErycibae caulis samples in order to classify samples collected from different regions. We hope that this assay can be readilyutilized as quality control method for Erycibae caulis.

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