리그노셀룰로오스의 혐기성 분해에 의한 탄수화물과 방향족 화합물의 축적속도 평가 (Evaluation of Accumulation Rates of Carbohydrates and Aromatic Compounds by Anaerobic Degradation of Lignocellulose)

서지정보

· 발행기관 : 한국수처리학회
· 수록지 정보 : 한국수처리학회지 / 30권 / 5호 / 3 ~ 14페이지
· 저자명 : 김석구

초록

This study evaluated the degradation properties of lignocellulose in methanogenic and sulfate-reducing environment. Carbohydrates and aromatic compounds, which are lignocellulosic degradation products, were analyzed and the accumulation rates were calculated. Seeding microorganisms, as well as leachate from a model landfill reactor composed of a methanogenic and sulfate-reducing environment that hadbeen operatingfor more than three years wereused. To calculate the accumulation rates of carbohydrates and aromatic compounds, serum vials cultured for 12 days were subjected to 3% (volume ratio) toluene. Toluene was used to selectively inhibit the activity of microorganisms while maintaining the activity of the extracellular degrading enzyme. Galactose, glucose, xylose and mannose produced from holocellulose such as office paper and newspaper in the presence of 3% toluene showed a linear increase in carbohydrate accumulation with time in a sulfate-reducing environment. The accumulation rates of glucose and xylose in the sulfate-reducing environment were faster than in the methanogenic environment, indicating that holocellulose decomposition was faster in the sulfate-reducing environment than in the methanogenic environment. For the lignin-derived aromatic compounds produced in newspapers in the presence of 3% toluene, 7 aromatic compounds were identified in the sulfate-reducing environment, compared to 3 aromatic compounds identified in the methanogenic environment. Accumulation rates of benzoic acid and vanillin were faster in the sulfate-reducing environment than in the methanogenic environment, indicating that lignin degradation was faster in the sulfate-reducing environment than in the methanogenic environment.

영어초록

This study evaluated the degradation properties of lignocellulose in methanogenic and sulfate-reducing environment. Carbohydrates and aromatic compounds, which are lignocellulosic degradation products, were analyzed and the accumulation rates were calculated. Seeding microorganisms, as well as leachate from a model landfill reactor composed of a methanogenic and sulfate-reducing environment that hadbeen operatingfor more than three years wereused. To calculate the accumulation rates of carbohydrates and aromatic compounds, serum vials cultured for 12 days were subjected to 3% (volume ratio) toluene. Toluene was used to selectively inhibit the activity of microorganisms while maintaining the activity of the extracellular degrading enzyme. Galactose, glucose, xylose and mannose produced from holocellulose such as office paper and newspaper in the presence of 3% toluene showed a linear increase in carbohydrate accumulation with time in a sulfate-reducing environment. The accumulation rates of glucose and xylose in the sulfate-reducing environment were faster than in the methanogenic environment, indicating that holocellulose decomposition was faster in the sulfate-reducing environment than in the methanogenic environment. For the lignin-derived aromatic compounds produced in newspapers in the presence of 3% toluene, 7 aromatic compounds were identified in the sulfate-reducing environment, compared to 3 aromatic compounds identified in the methanogenic environment. Accumulation rates of benzoic acid and vanillin were faster in the sulfate-reducing environment than in the methanogenic environment, indicating that lignin degradation was faster in the sulfate-reducing environment than in the methanogenic environment.

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