PCR-RFLP를 이용한 한우 Stearoyl-CoA Desaturase-1(SCD1)유전자의 SNP 및 경제형질 관련성 분석

서지정보

· 발행기관 : 강원대학교 동물자원공동연구소
· 수록지 정보 : 동물자원연구 / 23권 / 1호
· 저자명 : 박새롬, 홍민욱, 김훈, 이승규, 이영섭, 김진우, 송영한, 김기범, 오재돈, 이학교, 최정우, 이성진

목차

ABSTRACT Ι. 서론 II. 재료 및 방법 1. 공시재료 및 genomic DNA 추출 2. 공시재료의 DNA 동일성 검사 3. PCR primer 설계 및 증폭 4. SCD1 유전자 878T>C SNP의 유전자형 분석 5. 통계분석 III. 결과 및 고찰 1. 한우에서 SCD1 유전자의 PCR-RFLP 2. 한우 SCD1 유전자의 SNP와 한우 경제형질과의 관련성 분석 IV. 요약 사사 V. 인용문헌

영어초록

Stearoyl-CoA desaturase (SCD1) gene plays important role in fatty acid composition. In order to find marker-assisted selection (MAS) for improving the economic trait, this study was performed to identify the 878T>C single nucleotide polymorphism (SNP) on SCD1 gene. Three genotypes (CC, CT, and TT) were detected in 878T>C SNP of SCD1 gene from 103 Hanwoo population by polymorphism chain reaction-restriction fragment length polymorphism (PCR-RFLP) and economic traits were analyzed by general linear model. The frequency of allele C and T was 0.534 and 0.466, also the genotype frequency of CC, CT and TT was 0.252, 0.563, and 0.184, respectively in the Hanwoo population. The TT genotype of SCD1 gene showed a significantly higher measures (p<0.05) of carcass weight (CW) than CT, CC genotype. No significant association was detected between genotype and other economic trait (marbling, backfat thickness, and longissimus muscle area) in this study. The results revealed that SCD1 gene 878T>C SNP could be useful for effective MAS to increase the economic quality in Hanwoo population.

참고자료

· 없음