Staphylococcus aureus 정성시험

소개

"Staphylococcus aureus 정성시험"에 대한 내용입니다.

목차

1. Experimental purpose

2. Experimental overview
1) Principles
2) Summary

3. Experimental process
1) Materials
2) Methods

4. Experimental results
1) Results
2) Caculation process

5. Conclusions

6. Discussions

7. References

본문내용

* Experimental Title : Staphylococcus aureus 정성시험
1. Experimental purpose
- 식중독을 일으키는 대표적인 원인균 中 잠복기가 짧은 S. aureus를 정성시험하고자 한다. 이 실험은 표준균과 시료를 취하여 TSB 배지를 통해 증균배양하며, Baird-Parker agar 배지에서 분리배양 후 Gram stain, coagulase 시험을 통해 확인시험하여 시료에서 S. aureus 오염 유무를 판별하고자 한다. 또한 S. aureus 정성시험을 통해 원리 및 시험법을을 익히고자 한다.

2. Experimental overview
2. 1. Principles
- Staphylococcus aureus
S. aureus(황색포도상구균)는 Gram-positive 구균으로 한 쌍, 4개씩 또는 포도송이 모양으로 불규칙하게 배열되어 있다(Fig. 1). 균의 크기는 0.5-1.2um이며, 편모·포자·협막이 없고 비운동성이다. S. aureus는 자연계에 널리 분포되어 있을 뿐만 아니라 인간의 호흡계통 및 피부 등에서 자주 발견되며, Clostridium botulinum 식중독과 마찬가지로 식품 내 증식하면서 생성된 장독소(enterotoxin)를 섭취함으로써 발생되는 독소형 식중독균이다.

참고자료

· Lee, N. Y., Kim, Y. S., Shin, D. H. (2003). Growth Inhibitory Effects of Chloride Salts and zOrganic Acid Salts Against Food-Borne Microorganisms. Journal of the Korean Society zof Food Science and Nutrition, 32(8), 1233-1238.
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· Kessi, J., Turner, R. J., & Zannoni, D. (2022). Tellurite and Selenite: how can these two oxyanions be chemically different yet so similar in the way they are transformed to their metal forms by bacteria?. Biological research, 55(1), 17.
· Presentato Alessandro, Turner Raymond J., Vásquez Claudio C., Yurkov Vladimir, zZannoni Davide (2019) Tellurite-dependent blackening of bacteria emerges from the zdark ages. Environmental Chemistry 16, 266-288.
· D.V.D. Hemalika. (2021). Phospholipase enzymes as potential biomarker for SARS CoV-z2 virus. International Journal of Scientific and Research Publications(IJSRP) 11(1), 189-z197.
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· Brown, Wolf, J. M., Prados-Rosales, R., & Casadevall, A. (2015). Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi. Nature Reviews. Microbiology, 13(10), 620–630. Figure 1. Overall structure of S. aureus - https://en.wikipedia.org/wiki/Staphylococcus_aureus - https://www.pinterest.co.kr/pin/*************14438/ Figure 2. S. aureus on Baird-Parker agar medium - https://microbenotes.com/baird-parker-agar/ Figure 3. Overview model of tellurium oxyanion(s) exposure to prokaryotic cell - Kessi, J., Turner, R. J., & Zannoni, D. (2022). Tellurite and Selenite: how can these two zoxyanions be chemically different yet so similar in the way they are transformed to ztheir metal forms by bacteria?. Biological research, 55(1), 17.
· Figure 4. Functional properties and positive-reaction of lecithinase - D.V.D. Hemalika. (2021). Phospholipase enzymes as potential biomarker for SARS zCoV-2 virus. International Journal of Scientific and Research Publications(IJSRP) z11(1), 189-197. - https://www.vetbact.org/popup/popup.php?id=34&LANG=en Figure 6. Negative and positive Coagulase test - https://laboratorytests.org/coagulase-test/ Figure 7. Gram-negative and positive cell wall structures - Brown, L., Wolf, J., Prados-Rosales, R. et al. Through the wall: extracellular vesicles in zGram-positive bacteria, mycobacteria and fungi. Nat Rev Microbiol 13, 620–630 z(2015). Figure 8. Overview of the Gram staining method - https://theory.labster.com/reagents/ Figure 9. Difference in peptidoglycan thickness of gram-positive and negative bacteria - https://www.jove.com/kr/v/10092/gram-staining-of-bacteria-from-environmental-zsources Figure 10. Positive and negative oxidase test - https://thesciencenotes.com/biochemical-tests-for-the-identification-of-bacteria-ztypes-principle-procedure-and-examples/ Figure 11. Color change principle of TMPD reagent - https://asm.org/getattachment/00ce8639-8e76-4acb-8591-0f7b22a347c6/oxidase-ztest-protocol-3229.pdf