Enzyme Digestion, Ligation, Transformation 실험 레포트 (영문)

소개

대학교 생명과학 생화학 실험 과목 A+ 받은 영어 레포트입니다.
DNA digestion, ligation, transformation의 background와 실험 방법, 실험 결과, 토론 (Discussion)에 대해 상세하게 기록되어 있습니다.

목차

0. Abstract
1. Introduction
2. Method
3. Result
4. Discussion
5. Reference

본문내용

The main purpose of this experiment is to insert target genes into the vector. We did purification of PCR product using DNA purification kit. And then, we used XhoⅠ and HINDⅢ for restriction enzyme digestion. Again, we Purified this enzyme digested DNA to remove impurities. And using T4 DNA ligase, ligation of the enzyme cut plasmid and PCR products. In this step, we made two kinds of plate, control group and experimental group. The control group didn’t contain PCR products. Finally, transformation the ligated product into the competent cell (we used DH5

참고자료

· Jeremy M. Berg et al. 2019. Biochemistry. 9 th ed. W.H. Freeman and Company.
· Campbell et al. 2019. Campbell Biology. 10th ed. Pearson.
· “DNA ligase”. Solgent. 2019-08-16. http://www.solgent.com/sub03010202/view/id/36.
· “XhoI”. New England BioLabs Inc. https://international.neb.com/products/r0146xhoi#Product%20Information
· “HindIII”. New England BioLabs Inc. https://international.neb.com/products/r0104hindiii#Product%20Information
· “T4 DNA Ligase”. BIONEER. https://www.bioneer.co.kr/20-e-3061-cfg.html
· “Transformation of E.coli with pGAL(Blue Colony)”. Korea Scientifics.