저강도 맥동성 초음파 적용이 관절연골세포에 미치는 영향 (Effect of Low Intensity Pulsed Ultrasound in Rat Chondrocyte)

서지정보

· 발행기관 : 한의병리학회
· 수록지 정보 : 동의생리병리학회지 / 22권 / 5호 / 1262 ~ 1269페이지
· 저자명 : 김은정, 김계엽

초록

Low intensity pulsed ultrasound(LIPUS) is known to accelerate bone regeneration, but the precise cellular signaling mechanism is still unclear. The purpose if this study was to determine the effect of LIPUS on the signaling mechanism of rat chondrocyte. In the explant culture condition, there was inhibition effect of 1 W/㎠ intensity LIPUS on chondrocytes proliferation but chondrocytes proliferation was increased at 0.25 W/㎠ intensity. In addition, western blot analysis of MAPKs showed that LIPUS increased ERK1/2 activity from the 10 min treatment of LIPUS. Hydrogen peroxide(H2O2), resulted in a time- and dose-dependent cell proliferation, which was largely attributed to apoptosis. H2O2 treatment caused marked sustained nucleus condensation in Hoechst stain. LIPUS and H2O2 activates phosphorylation of p-ERK1/2 and PD 98059(10-5M) blocked the effect of LIPUS and H2O2. Moreover, the synergistic phosphorylation of p44/42 MAPK by H2O2, LIPUS was selectively inhibited by PD 98059, ERK1/2 inhibitor. In order to determine whether the increase in cell proliferation caused by H2O2 and LIPUS could be explained by changes in the level of the prostaglandin E2. Our study demonstrated that LIPUS stimulate the cell proliferation via activated phosphorylation of ERK1/2 in condrocyte. LIPUS has anabolic effects on rat cartilage in explant cultures, indicating a potential important method for the treatment of osteoarthritic cartilarge.

영어초록

Low intensity pulsed ultrasound(LIPUS) is known to accelerate bone regeneration, but the precise cellular signaling mechanism is still unclear. The purpose if this study was to determine the effect of LIPUS on the signaling mechanism of rat chondrocyte. In the explant culture condition, there was inhibition effect of 1 W/㎠ intensity LIPUS on chondrocytes proliferation but chondrocytes proliferation was increased at 0.25 W/㎠ intensity. In addition, western blot analysis of MAPKs showed that LIPUS increased ERK1/2 activity from the 10 min treatment of LIPUS. Hydrogen peroxide(H2O2), resulted in a time- and dose-dependent cell proliferation, which was largely attributed to apoptosis. H2O2 treatment caused marked sustained nucleus condensation in Hoechst stain. LIPUS and H2O2 activates phosphorylation of p-ERK1/2 and PD 98059(10-5M) blocked the effect of LIPUS and H2O2. Moreover, the synergistic phosphorylation of p44/42 MAPK by H2O2, LIPUS was selectively inhibited by PD 98059, ERK1/2 inhibitor. In order to determine whether the increase in cell proliferation caused by H2O2 and LIPUS could be explained by changes in the level of the prostaglandin E2. Our study demonstrated that LIPUS stimulate the cell proliferation via activated phosphorylation of ERK1/2 in condrocyte. LIPUS has anabolic effects on rat cartilage in explant cultures, indicating a potential important method for the treatment of osteoarthritic cartilarge.

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