녹각교가 난소절제 흰쥐에서 골수기질 간세포의 증식 및 분화에 미치는 영향 (Effects of old antler on proliferation and differentiation of bone marrow stromal stem cells in ovariectomized rats)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 1권 / 2호 / 184 ~ 189페이지
· 저자명 : 원영준, 안혜은, 문경호, 민병현, 황태숙, 서창국, 박소라

초록

Herbal medicines have been claimed to have beneficial effects for the fracture healing and bone formation.
Extracts of antler and old antler were known to have effects on the immune modulation, lowering of cholesterol, correction of anemia, detoxification of hepatic injuries, and the growth. However, beneficial effects on the bone metabolism were not determined. In order to investigate the effects of bone metabolism, extracts of old antler were used for in vivo and in vitro study. Eight rats (2-month-old female Sprague-Dawley rats) were placed on Chow diet and served as control (C), and eight rats were got Chow diet with old antler (C+OA) for 12 weeks. Eight rats were done ovariectomy for 4 weeks and had Chow diet (OVX), and another eight ovariectomized rats had Chow diet with old antler (OVX+OA). Sixteen rats were fed Chow diet with 0.4g/kg old antler daily. After 12 weeks, all experimental rats were sacrified for removing the bone marrow stromal stem cells. Bone marrow stromal stem cells rats were cultured and resuspend α-MEM containing 10 % heat inactivated fetal bovine serum (FBS) and antibiotics. Total colony forming unit-fibroblasts (CFU-Fs) and alkaline phosphate stained CFU-Fs cells were counted at 12 days after cell culture. In addition, mineralized nodule was also measured by light microscope at 21 days after cell culture. Total CFU-Fs colony counts were not significant difference among the study groups. Alkaline phosphatase positive colonies of ovariectomized rats with treating of extracts of old antler were significantly increase than those of ovariectomized with Chow diet. The amounts of mineralized nodule of OVX+OA group were significantly increase than those of OVX group. In conclusion, osteogenic differentiation of mesenchymal stem cells were enhanced after treated with extracts of old antler on ovariectomized rats.

영어초록

Herbal medicines have been claimed to have beneficial effects for the fracture healing and bone formation.
Extracts of antler and old antler were known to have effects on the immune modulation, lowering of cholesterol, correction of anemia, detoxification of hepatic injuries, and the growth. However, beneficial effects on the bone metabolism were not determined. In order to investigate the effects of bone metabolism, extracts of old antler were used for in vivo and in vitro study. Eight rats (2-month-old female Sprague-Dawley rats) were placed on Chow diet and served as control (C), and eight rats were got Chow diet with old antler (C+OA) for 12 weeks. Eight rats were done ovariectomy for 4 weeks and had Chow diet (OVX), and another eight ovariectomized rats had Chow diet with old antler (OVX+OA). Sixteen rats were fed Chow diet with 0.4g/kg old antler daily. After 12 weeks, all experimental rats were sacrified for removing the bone marrow stromal stem cells. Bone marrow stromal stem cells rats were cultured and resuspend α-MEM containing 10 % heat inactivated fetal bovine serum (FBS) and antibiotics. Total colony forming unit-fibroblasts (CFU-Fs) and alkaline phosphate stained CFU-Fs cells were counted at 12 days after cell culture. In addition, mineralized nodule was also measured by light microscope at 21 days after cell culture. Total CFU-Fs colony counts were not significant difference among the study groups. Alkaline phosphatase positive colonies of ovariectomized rats with treating of extracts of old antler were significantly increase than those of ovariectomized with Chow diet. The amounts of mineralized nodule of OVX+OA group were significantly increase than those of OVX group. In conclusion, osteogenic differentiation of mesenchymal stem cells were enhanced after treated with extracts of old antler on ovariectomized rats.

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