HaCaT 피부각질세포에서 들깻잎 추출물의 산화적 스트레스에 대한 항산화 효과 (Protective Effects of Perilla frutescens Britt var. japonica Extracts from Oxidative Stress in Human HaCaT Keratinocytes)

서지정보

· 발행기관 : 한국식품영양과학회
· 수록지 정보 : 한국식품영양과학회지 / 42권 / 2호 / 161 ~ 167페이지
· 저자명 : 지나, 송가락, 길정하, 박건영

초록

The aim of this study was to investigate the protective effects of methanolic extract from perilla (Perilla frutescens Britt var. japonica) leaves (PLME) on oxidative injury from hydrogen peroxide (H2O2) in human HaCaT keratinoctyes. Cells were co-incubated with various concentrations (0～200 μg/mL) of PLME for 24 hr, and then exposed to H2O2 (500 μM) for 4 hr. H2O2 significantly decreased cell viability (p<0.05). However, PLME provided protection from H2O2-induced HaCaT cell oxidation in a dose-dependent manner. To further investigate the protective effects of PLME on H2O2-induced oxidative stress in HaCaT cells, the cellular levels of lipid peroxidation, and antioxidant enzymes (including superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and catalase (CAT)) were measured. PLME decreased cellular levels of lipid peroxidation, and also increased the activities of antioxidant enzymes. In addition, the antioxidant activities of PLME were also determined by DPPH and hydroxyl (․OH) radical scavenging assay, and major antioxidant compounds of PLME were measured by colorimetric methods. DPPH and ․OH radical scavenging activities of PLME increased in a dose dependent manner and was similar to the DPPH scavenging activity of ascorbic acid at 50 μg/mL; however PLME activities were stronger than ascorbic acid (50 μg/mL) in the ․OH scavenging assay. The amounts of antioxidant compounds, including total polyphenolics, total flavonoids, and total ascorbic acid from PLME were 52.2±1.1 mg gallic acid (GAE)/g, 33.7±4.7 mg rutin (RUE)/g, and 17.0±0.5 mg ascorbic acid (AA)/g, respectively. These results suggest that PLME has a strong free radical-scavenging activity and a protective effect against H2O2-induced oxidative stress in the keratinocytes.

영어초록

The aim of this study was to investigate the protective effects of methanolic extract from perilla (Perilla frutescens Britt var. japonica) leaves (PLME) on oxidative injury from hydrogen peroxide (H2O2) in human HaCaT keratinoctyes. Cells were co-incubated with various concentrations (0～200 μg/mL) of PLME for 24 hr, and then exposed to H2O2 (500 μM) for 4 hr. H2O2 significantly decreased cell viability (p<0.05). However, PLME provided protection from H2O2-induced HaCaT cell oxidation in a dose-dependent manner. To further investigate the protective effects of PLME on H2O2-induced oxidative stress in HaCaT cells, the cellular levels of lipid peroxidation, and antioxidant enzymes (including superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and catalase (CAT)) were measured. PLME decreased cellular levels of lipid peroxidation, and also increased the activities of antioxidant enzymes. In addition, the antioxidant activities of PLME were also determined by DPPH and hydroxyl (․OH) radical scavenging assay, and major antioxidant compounds of PLME were measured by colorimetric methods. DPPH and ․OH radical scavenging activities of PLME increased in a dose dependent manner and was similar to the DPPH scavenging activity of ascorbic acid at 50 μg/mL; however PLME activities were stronger than ascorbic acid (50 μg/mL) in the ․OH scavenging assay. The amounts of antioxidant compounds, including total polyphenolics, total flavonoids, and total ascorbic acid from PLME were 52.2±1.1 mg gallic acid (GAE)/g, 33.7±4.7 mg rutin (RUE)/g, and 17.0±0.5 mg ascorbic acid (AA)/g, respectively. These results suggest that PLME has a strong free radical-scavenging activity and a protective effect against H2O2-induced oxidative stress in the keratinocytes.

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