젤방사성형법에 의한 혈관용 스캐폴드의 제조 및 특성 (Study on the tubular scaffold fabricated by gel-spinning)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 3권 / 1호 / 39 ~ 45페이지
· 저자명 : 정은나, 김상헌, 권재현, 정민섭, 박인수, 고영건, 한성식, 김영하, 김수현

초록

We developed a biodegradable porous scaffold fabricated by a gel spinning method using a biodegradable
and elastic polymer, poly(L-lactide-co-ε-caprolactone) (PLCL) (50:50) for vascular grafts application. To characterize
the scaffold, we tested fiber thickness, SEM, cell seeding efficiency, tensile properties, and elasticity. In seeding
test, we used bone marrow mononuclear cells isolated from rabbit. Furthermore, we examined tissue ingrowth activities
for the scaffolds implanted in nude mice. The fiber thickness of the gel-spun scaffolds increased as the concentration
of polymer solution increased. In mechanical properties such as tensile strength and elasticity, the gel-spun
scaffolds were superior to scaffolds fabricated by a extrusion technique. In addition, the cell seeding efficiency of
the scaffolds was higher than that of other scaffolds fabricated by compared methods. In implantation experiments,
the scaffolds showed a good tissue ingrowth through open spaces between micro fibers. In conclusion, gel-spun
tubular scaffolds can be a good candidate as scaffolds for cardiovascular tissue engineering.

영어초록

We developed a biodegradable porous scaffold fabricated by a gel spinning method using a biodegradable
and elastic polymer, poly(L-lactide-co-ε-caprolactone) (PLCL) (50:50) for vascular grafts application. To characterize
the scaffold, we tested fiber thickness, SEM, cell seeding efficiency, tensile properties, and elasticity. In seeding
test, we used bone marrow mononuclear cells isolated from rabbit. Furthermore, we examined tissue ingrowth activities
for the scaffolds implanted in nude mice. The fiber thickness of the gel-spun scaffolds increased as the concentration
of polymer solution increased. In mechanical properties such as tensile strength and elasticity, the gel-spun
scaffolds were superior to scaffolds fabricated by a extrusion technique. In addition, the cell seeding efficiency of
the scaffolds was higher than that of other scaffolds fabricated by compared methods. In implantation experiments,
the scaffolds showed a good tissue ingrowth through open spaces between micro fibers. In conclusion, gel-spun
tubular scaffolds can be a good candidate as scaffolds for cardiovascular tissue engineering.

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