고추 Shed 소포자 배양시 2-Step 재분화 방법을 이용한 식물체 생산 효율 증대 기술 (Increasing Plant Production Efficiency Using a 2-Step Regeneration Method in Shed Microspore Culture Process of Pepper(Capsicum annuum L.))

서지정보

· 발행기관 : 한국농수산대학교 국제농업교육센터
· 수록지 정보 : 현장농수산연구지 / 26권 / 4호 / 69 ~ 76페이지
· 저자명 : 박은준, 안율균, 권덕호, 양은영

초록

This study was conducted to improve the plant regeneration rate, which is a problem in shed microspore culture of peppers, by optimizing the post-embryogenesis culture environment and evaluating its effects. In shed microspore culture, after six weeks of cultivation, there is a significant difference in the embryogenesis efficiency of microspore embryos. This trend is not observed in isolated microspore culture but appears specifically in anther culture, where anthers are extracted from individual flower buds for culture, and in shed-microspore culture. Additionally, when microspore embryos induced through shed microspore culture are transferred to general regeneration containers (100×40mm), many embryos fail to develop into plants and die. To resolve this issue, a two-step regeneration process was established. After six weeks of culture, microspore embryos were first transferred to 90×20mm containers, and then after 2–4 weeks, they were moved to 100×40mm containers. As a result, it was confirmed that normal haploid/doubled haploid plants could be obtained. While there were slight varietal differences in plant production using the two-step regeneration culture method, both HSM003 and HSM005 varieties showed significantly improved regeneration rates. This method is expected to enable the acquisition of a large number of haploids and doubled haploids derived from microspores not only through isolated microspore culture but also through shed-microspore culture.

영어초록

This study was conducted to improve the plant regeneration rate, which is a problem in shed microspore culture of peppers, by optimizing the post-embryogenesis culture environment and evaluating its effects. In shed microspore culture, after six weeks of cultivation, there is a significant difference in the embryogenesis efficiency of microspore embryos. This trend is not observed in isolated microspore culture but appears specifically in anther culture, where anthers are extracted from individual flower buds for culture, and in shed-microspore culture. Additionally, when microspore embryos induced through shed microspore culture are transferred to general regeneration containers (100×40mm), many embryos fail to develop into plants and die. To resolve this issue, a two-step regeneration process was established. After six weeks of culture, microspore embryos were first transferred to 90×20mm containers, and then after 2–4 weeks, they were moved to 100×40mm containers. As a result, it was confirmed that normal haploid/doubled haploid plants could be obtained. While there were slight varietal differences in plant production using the two-step regeneration culture method, both HSM003 and HSM005 varieties showed significantly improved regeneration rates. This method is expected to enable the acquisition of a large number of haploids and doubled haploids derived from microspores not only through isolated microspore culture but also through shed-microspore culture.

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