두릅 열수추출물의 항염 및 항헬리코박터 효과 (Anti-inflammatory and anti-Helicobacter effects of the Aralia elata hot-water extract)

서지정보

· 발행기관 : 한국식품저장유통학회
· 수록지 정보 : Food Science and Preservation / 28권 / 2호 / 279 ~ 287페이지
· 저자명 : 이세원, 이정호

초록

Aralia elata contains several bioactive compounds, such as saponin, oleanolic acid, and beta-carotene, which are known widely to control blood pressure and diabetes. In this study, we investigated the anti-Helicobacter pylori, antioxidant, anti-inflammatory, and cytotoxic effects of the extract of A. elata which was procured from Sunchang-gun, South Korea. The extract was prepared using water bath at 80℃ for 5 h. The total polyphenolics content in A. elata hot-water extract was 186.8±2.7 mgGAE/g, and the total flavonoid content was 81.9±1.5 mgQE/g. In addition, the extract exhibited anti-Helicobacter pylori activity and the growth of the bacteria was decreased with increasing concentration of the extract. SC50 value of DPPH radical scavenging activity was 3,274.7±47.7 μg/mL, and ABTS radical scavenging activity was 2,660.1±50.3 μg/mL. Furthermore, A. elata hot-water extract reduced the production of nitric oxide (NO), TNF-α, IL-1β, and IL-6 in LPS-stimulated RAW264.7 cells. Cell viability assay revealed no cytotoxicity in RAW264.7 cells even at a concentration of 100 μg/mL. The results confirmed that A. elata hot-water extract could be used as an antioxidant, antibacterial, and anti-inflammatory agent.

영어초록

Aralia elata contains several bioactive compounds, such as saponin, oleanolic acid, and beta-carotene, which are known widely to control blood pressure and diabetes. In this study, we investigated the anti-Helicobacter pylori, antioxidant, anti-inflammatory, and cytotoxic effects of the extract of A. elata which was procured from Sunchang-gun, South Korea. The extract was prepared using water bath at 80℃ for 5 h. The total polyphenolics content in A. elata hot-water extract was 186.8±2.7 mgGAE/g, and the total flavonoid content was 81.9±1.5 mgQE/g. In addition, the extract exhibited anti-Helicobacter pylori activity and the growth of the bacteria was decreased with increasing concentration of the extract. SC50 value of DPPH radical scavenging activity was 3,274.7±47.7 μg/mL, and ABTS radical scavenging activity was 2,660.1±50.3 μg/mL. Furthermore, A. elata hot-water extract reduced the production of nitric oxide (NO), TNF-α, IL-1β, and IL-6 in LPS-stimulated RAW264.7 cells. Cell viability assay revealed no cytotoxicity in RAW264.7 cells even at a concentration of 100 μg/mL. The results confirmed that A. elata hot-water extract could be used as an antioxidant, antibacterial, and anti-inflammatory agent.

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