적양배추 추출물의 항산화 및 항염증 활성 (Antioxidant and Anti-inflammation Activity of Red Cabbage Extract)

서지정보

· 발행기관 : (사)한국조리학회
· 수록지 정보 : Culinary Science & Hospitality Research / 20권 / 2호 / 16 ~ 26페이지
· 저자명 : 하현주, 이춘복

초록

본 연구에서는 생리활성이 우수한 것으로 알려진 적양배추를 이용하여 에탄올 및 열수 추출을하여 마크로파지 세포에 LPS로 유도하여 염증성질환 모델에서 세포생존, NO생성 억제, 단백질단계에서 iNOS와 COX-2의 발현을 분석하였고항산화 활성 측정을 위해 ABTS 라디칼 소거능과FRAP 활성을 확인하였다. 각 추출물의 세포생존,NO생성 및 단백질 발현 분석에서 농도 의존적으로 LPS에 대해 억제 및 저해 효능을 확인하였고특히 0.25, 0.5 그리고 1.0 mg/mL에서 유의적인차이를 확인하였다. ABTS 라디칼 소거 및 FRAP활성은 합성 항산화제인 BHT보다 활성이 좋지않았지만 천연 항산화제로써의 가능성을 확인하였고 훌륭한 식품소재로서 기능성 식품 및 각 종질병에 대한 예방의 목적으로 활용이 가능할 것으로 사료된다.

영어초록

This study investigated the anti-inflammatory and antioxidnat effects of red cabbage extracts on RAW264.7cells. Cell toxicity was determined by MTT assay. We evaluated the anti-inflammatory effects of red cabbageextracts by measuring nitric oxide (NO), inducible NOS (iNOS) production, and cyclooxygenase-2 (COX-2)expression by Western blotting. Ethanolic and water extracts (0.25, 0.5, and 1.0 mg/mL) significantlysuppressed LPS-stimulated production of NO. Two kinds of extracts reduced the expression of iNOS andCOX-2 proteins. The present results show that red cabbage extract has potent anti-inflammatory effects onRAW264.7 cells. In addition, two kinds of extracts as well as various antioxidant activities such as2,2'-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid)(ABTS) radical scavenging activity, ferric reducingantioxidant power(FRAP). The total polyphenol and flavonoid contents of the ethanolic and water extractsfrom red cabbage were 18.699 ± 0.87 and 11.174 ± 4.86 mg GAE/g extract, respectively, and 7.782 ± 2.23and 15.608 ± 3.54 mg CE/g extract. The ABTS radical scavenging activities of the ethanolic and waterextracts and BHT were 0.269 ± 0.12, 0.212 ± 0.22 and 1.235 ± 0.07 mM Trolox equivalent/mg extract,respectively. The FRAP values of the extracts were similar to those of BHT, which were used as a positivecontrol. Therefore, red cabbage extract is considered as a good food material of functional foods forprevention against various diseases.

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