산딸나무(Cornus kousa) 열매 추출물의 생리활성 (Biological Activities of Extracts from Cornus kousa Fruit)

서지정보

· 발행기관 : 한국응용생명화학회
· 수록지 정보 : Journal of Applied Biological Chemistry / 58권 / 4호 / 317 ~ 323페이지
· 저자명 : 이은호, 이선호, 조영제

초록

산딸나무 열매로부터 phenolic compounds를 추출 후 생리활성을 검정하여 기능성 소재로 활용가능성을 살펴보았다. 산딸나무열매에 함유된 페놀성 물질은 물과 40% ethanol을 용매로 하여 추출하였을 때 각각 7.04, 4.47 mg/g 함량을 나타내었다. 추출물의 phenolic 농도를 50−200 μg/mL로 조절하여 1,1-diphenyl- 2-picrylhydrazyl를 측정한 결과, 물 추출물과 ethanol 추출물 50 μg/mL phenolics 농도에서 각각 84, 86%였고, 2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid radical decolorization은100 μg/mL phenolics 농도의 물 추출물과 ethanol 추출물에서각각 84%, 95%였다. Antioxidant protection factor는 50 μg/ mL phenolics 농도에서 물 추출물과 ethanol 추출물에서 각각1.93와 1.82 PF로 측정되었으며, TBARs값은 150 μg/mL phenolics 농도에서 물 추출물이 69%, ethanol 추출물에서 89%를 나타내었다. Xanthine oxidase 저해능은 물 추출물과 ethanol 추출물에서 각각 34, 60%로 측정되었으며, α-glucosidase 저해능은 물추출물과 ethanol 추출물에서 각각 29, 87%의 높은 효능을 보였다. Tyrosinase 저해능을 측정한 결과 ethanol 추출물에서만19%의 효능을 나타내었다. Collagenase 저해능 측정 결과 200 μg/mL phenolics 농도에서 물 추출물과 ethanol 추출물이 각각53, 77%의 높은 저해력을 나타내어 주름개선효과가 높았다. 염증억제효과로서 hyaluronidae 저해활성을 측정한 결과, 200 μg/ mL phenolic 농도의 물 추출물에서 34%의 염증억제효과를 나타내었다. 이러한 결과로 보아 산딸나무 열매추출물은 항산화, 통풍억제, 당분해 억제, 주름개선효과, 염증억제 등의 기능성 소재로서 활용이 가능할 것으로 판단되었다.

영어초록

The extracted phenolic compounds from Cornus kousa fruit for biological activities as functional resources were examined. The phenolic compounds which were extracted with water and 40% ethanol from Cornus kousa fruit were 7.04±0.27 and 4.47±0.18mg/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity of water and ethanol extracts were 84% and 86% at 50 μg/mL phenolics, respectively. The 2,2'- Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid radical decolorization activity of water and ethanol extracts were 84 and 95% at 100 μg/ mL phenolics, respectively. Antioxidant protection factor in water and ethanol extracts at 50 μg/mL phenolics were 1.93 and 1.82 PF, respectively. Thiobarbituric acid reactive substance were 69% in water extracts and 89% in ethanol extracts at 150 μg/mL phenolics.
The inhibition activity on xanthine oxidase in water and ethanol extracts was 34 and 60%, respectively. The inhibition activity on α-glucosidase was 29% in water extracts and 87% in ethanol extracts. The tyrosinase inhibitory activity was 19% in ethanol extracts. The collagenase inhibition activity of anti-wrinkle effect showed an excellent wrinkle improvement effect as 53% in water extracts and 77% in ethanol extracts at 200 μg/mL phenolics. The hyaluronidase inhibition activity as antiinflammation effect of water extracts was confirmed to 34% of inhibition at 200 μg/mL phenolic. The results can be expected extracts from Cornus kousa fruit to use as functional resource for antioxidant, antigout, inhibitor of carbohydrate degradation, antiwrinkle activity and antiinflammation activity.

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