毛棶枝葉(Cornus walteri Wanger) 추출물이 비만 쥐의 지질강하, 항산화효과 및 전염증성 cytokines 농도에 미치는 영향 (Effects of Stem Bark Extracts of Cornus walteri Wanger on the Lipid Lowering, Anti-oxidative Activity and Concentration of Proinflammatory Cytokines in Rat Fed High Fat Diet)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 19권 / 4호 / 59 ~ 78페이지
· 저자명 : 박원형, 차윤엽

초록

Objective:
This study was designed to examine the effects of stem bark extracts of Cornus walteri Wanger on the lipid lowering, anti-oxidative activity and concentration of proinflammatory cytokines were investigated in hyperlipidemic rat.

Methods:
Male rats weighing 195.21±5.85g fed high fat diet for 8 weeks and 40 rats(above 400g) were divided into 4 groups. Each of 10 rats was devided into a control group and experimental groups. We fed a control group of rats a basal diet and administered normal saline(100 ㎎/㎏, 1 time/1 day) for 4 weeks. And we fed each experimental group of rats Basal diet and administered an extract of Cornus walteri Wanger(100 ㎎/㎏, 200 ㎎/㎏, 300 ㎎/㎏, 1 time/1 day) for 4 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. We measured lipid of plasma and liver, concentration of proinflammatory cytokines, anti-oxidative activity and gene expression.

Results:
1. Concentration of plasma FFA showed a tendency to decrease in Cornus walteri Wanger ext. groups. Concentration of plasma TG showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group.
2. Concentration of plasma total cholesterol showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group. Concentration of plasma LDL-cholesterol showed a tendence to decrease in Cornus walteri Wanger ext. groups. Concentration of plasma HDL-cholesterol showed a significantly increment in the 300㎎/㎏ Cornus walteri Wanger ext. group.
3. Concentration of liver total cholesterol showed a tendence to decrease in Cornus walteri Wanger ext. groups. Concentration of liver TG showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group.
4. Concentration of plasma and liver TBARS showed a tendence to decrease in Cornus walteri Wanger ext. groups.
5. The values of GSH-Px, SOD and CAT activity showed a significantly increment in the 300 ㎎/㎏ Cornus walteri Wanger ext. group than that of control group.
6. The values of plasma AST and ALT activity showed no significantly different in all treatment groups.
7. Concentration of plasma IL-1β, IL-6 and TNF-α showed a tendency to decrease in the Cornus walteri Wanger ext. groups. However the concentration of IL-10 in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. groups showed a significantly increment than that of control group.
8. Concentration of liver IL-1β, TNF-α and IL-10 showed no significantly difference in among treatment groups. However concentration of IL-6 in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. groups showed a significantly decrement than that of control group.
9. In the analysis of RT-PCR, gene expression of TNF-α, Apo-B, Apo-E and leptin in the Cornus walteri Wanger ext. groups showed a lower expression than that of control group.
10. The ratio of TNF-α, Apo-E and leptin expression per β-actin expression in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. showed a significantly decrement than that of control group. The ratio of Apo-B expression per β-actin expression in the 300 ㎎/㎏ Cornus walteri Wanger ext. showed a significantly decrement than that of control group.

Conclusions:
According to above results, in lowering lipid effect, antioxidative activity and antiinflammatory effect, the Cornus walteri Wanger ext. gives positive effect.

영어초록

Objective:
This study was designed to examine the effects of stem bark extracts of Cornus walteri Wanger on the lipid lowering, anti-oxidative activity and concentration of proinflammatory cytokines were investigated in hyperlipidemic rat.

Methods:
Male rats weighing 195.21±5.85g fed high fat diet for 8 weeks and 40 rats(above 400g) were divided into 4 groups. Each of 10 rats was devided into a control group and experimental groups. We fed a control group of rats a basal diet and administered normal saline(100 ㎎/㎏, 1 time/1 day) for 4 weeks. And we fed each experimental group of rats Basal diet and administered an extract of Cornus walteri Wanger(100 ㎎/㎏, 200 ㎎/㎏, 300 ㎎/㎏, 1 time/1 day) for 4 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. We measured lipid of plasma and liver, concentration of proinflammatory cytokines, anti-oxidative activity and gene expression.

Results:
1. Concentration of plasma FFA showed a tendency to decrease in Cornus walteri Wanger ext. groups. Concentration of plasma TG showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group.
2. Concentration of plasma total cholesterol showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group. Concentration of plasma LDL-cholesterol showed a tendence to decrease in Cornus walteri Wanger ext. groups. Concentration of plasma HDL-cholesterol showed a significantly increment in the 300㎎/㎏ Cornus walteri Wanger ext. group.
3. Concentration of liver total cholesterol showed a tendence to decrease in Cornus walteri Wanger ext. groups. Concentration of liver TG showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group.
4. Concentration of plasma and liver TBARS showed a tendence to decrease in Cornus walteri Wanger ext. groups.
5. The values of GSH-Px, SOD and CAT activity showed a significantly increment in the 300 ㎎/㎏ Cornus walteri Wanger ext. group than that of control group.
6. The values of plasma AST and ALT activity showed no significantly different in all treatment groups.
7. Concentration of plasma IL-1β, IL-6 and TNF-α showed a tendency to decrease in the Cornus walteri Wanger ext. groups. However the concentration of IL-10 in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. groups showed a significantly increment than that of control group.
8. Concentration of liver IL-1β, TNF-α and IL-10 showed no significantly difference in among treatment groups. However concentration of IL-6 in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. groups showed a significantly decrement than that of control group.
9. In the analysis of RT-PCR, gene expression of TNF-α, Apo-B, Apo-E and leptin in the Cornus walteri Wanger ext. groups showed a lower expression than that of control group.
10. The ratio of TNF-α, Apo-E and leptin expression per β-actin expression in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. showed a significantly decrement than that of control group. The ratio of Apo-B expression per β-actin expression in the 300 ㎎/㎏ Cornus walteri Wanger ext. showed a significantly decrement than that of control group.

Conclusions:
According to above results, in lowering lipid effect, antioxidative activity and antiinflammatory effect, the Cornus walteri Wanger ext. gives positive effect.

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