돼지 자궁 세포의 3차원 배양이 Plasminogen 활성과 수정란 발육에 미치는 영향 (Effect of Three Dimensional Culture of Porcine Endometrial Cells on Their Plasminogen Activity and Pre-implantation Embryo Development after Co-culture)

서지정보

· 발행기관 : 사단법인 한국동물생명공학회
· 수록지 정보 : 한국동물생명공학회지 / 29권 / 3호 / 207 ~ 219페이지
· 저자명 : 이상희, 황보용, 차혜진, 김수진, 김민경, 정희태, 양부근, 박춘근

초록

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used variousexperiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3Dcultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrialand myometrial cells. The most rapid growth of stromal cells were confirmed in 2.5×105 cells/ml and gel matrixcontaining 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) werehigher than without hCG, but, there are not significant difference among the treatment. On the other hand, expressionof uPA after treatment of IL-1β (0.1, 1, 10 and 100 ng/ml) were higher than without IL-1β, but, there are not significantdifference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PAactivity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS andendometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavagerates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culturesystem. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivophysiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that3D culture system with embryo co-culture need continuous research.

영어초록

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used variousexperiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3Dcultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrialand myometrial cells. The most rapid growth of stromal cells were confirmed in 2.5×105 cells/ml and gel matrixcontaining 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) werehigher than without hCG, but, there are not significant difference among the treatment. On the other hand, expressionof uPA after treatment of IL-1β (0.1, 1, 10 and 100 ng/ml) were higher than without IL-1β, but, there are not significantdifference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PAactivity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS andendometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavagerates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culturesystem. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivophysiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that3D culture system with embryo co-culture need continuous research.

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