난알부민 유도 알레르기 면역반응에 대한 당삼(黨參)에탄올추출물의 효능 연구 (Effects of the ethanol extract of Codonopsis Pilosulae Radix on ovalbumin-induced allergic responses in mice)

서지정보

· 발행기관 : 대한본초학회
· 수록지 정보 : 대한본초학회지 / 28권 / 2호 / 9 ~ 15페이지
· 저자명 : 강석용, 정진기, 이상국, 이승호, 박용기

초록

Objectives : The root of Codonopsis pilosula (Fr.) Nannf. (Codonopsis Pilosulae Radix) has been traditionallyused as a oriental medicine with an anti-thrombotic, antidiabetic, anticancer, and anti-gastric ulcer effects andimmunological adjuvant. In this study, we investigated the effect of 70% ethanol extract of Codonopsis PilosulaeRadix (CPR-E) on ovalbumin (OVA)-induced allergic responses in mice.
Methods : Mice were sensitized (1, 8, and 15 days) with OVA and airway challenged(22, 24, 26, 28, and 30days) to induced allergic responses. CPR-E extract at doses of 50 and 100 ㎎/㎏/body weight was orallyadministered from days 21 to 30 consecutively. The levels of allergic mediators such as histamine, OVA-specificimmunoglobulin (Ig) E, and Th1/Th2 cytokines such as IFN-γ and IL-4 were measured in the sera of mice byELISA. The histological change of lung tissue was observed by hematoxylin and eosin (H&E) staining.
Results : CPR-E extract significantly decreased the serum levels of histamine, OVA-specific IgE, and IL-4compared with those of OVA control group, but significantly increased the serum level of IFN-γ. Based onH&E staining, CPR-E extract inhibited the infiltration of inflammatory cells into lung tissues with histologicalchanges.
Conclusions : These results indicate that CPR-E extract has anti-inflammatory and anti-allergic responsesthrough regulating the cytokine balance, suggesting that the extract may be useful for the treatment of allergicinflammatory diseases such as bronchial asthma and allergic rhinitis.

영어초록

Objectives : The root of Codonopsis pilosula (Fr.) Nannf. (Codonopsis Pilosulae Radix) has been traditionallyused as a oriental medicine with an anti-thrombotic, antidiabetic, anticancer, and anti-gastric ulcer effects andimmunological adjuvant. In this study, we investigated the effect of 70% ethanol extract of Codonopsis PilosulaeRadix (CPR-E) on ovalbumin (OVA)-induced allergic responses in mice.
Methods : Mice were sensitized (1, 8, and 15 days) with OVA and airway challenged(22, 24, 26, 28, and 30days) to induced allergic responses. CPR-E extract at doses of 50 and 100 ㎎/㎏/body weight was orallyadministered from days 21 to 30 consecutively. The levels of allergic mediators such as histamine, OVA-specificimmunoglobulin (Ig) E, and Th1/Th2 cytokines such as IFN-γ and IL-4 were measured in the sera of mice byELISA. The histological change of lung tissue was observed by hematoxylin and eosin (H&E) staining.
Results : CPR-E extract significantly decreased the serum levels of histamine, OVA-specific IgE, and IL-4compared with those of OVA control group, but significantly increased the serum level of IFN-γ. Based onH&E staining, CPR-E extract inhibited the infiltration of inflammatory cells into lung tissues with histologicalchanges.
Conclusions : These results indicate that CPR-E extract has anti-inflammatory and anti-allergic responsesthrough regulating the cytokine balance, suggesting that the extract may be useful for the treatment of allergicinflammatory diseases such as bronchial asthma and allergic rhinitis.

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