고성능 액체 크로마토그래피에서 아미노산과 이들 에스테르의 플루오레닐메톡시카르보닐 유도체의 자외선과 형광 검출에서의 동시 광학분리 (Simultaneous Enantiomer Separation of α-Amino Acids and Their Esters as Fluorenylmethoxycarbonyl Derivatives under UV and Fluorescence Detection by High Performance Liquid Chromatograph)

서지정보

· 발행기관 : 한국생물공학회
· 수록지 정보 : KSBB Journal / 30권 / 4호 / 197 ~ 201페이지
· 저자명 : 이스람 포크롤, 이원재

초록

Liquid chromatographic enantiomer separation of α-amino acids and their methyl and ethyl esters as fluorenylmethoxycarbonyl (FMOC) derivatives was performed using a recently developed chiral column (Chiralpak IE) based on polysaccharide derivative under simultaneous UV detection and fluorescence detection. The degree of enantiomer separation of α-amino acid esters as FMOC derivatives is generally higher than that of the corresponding α-amino acids. Especially, α-amino acid methyl esters showed the greatest enantioseparation.
As this method developed in this study can be applied to determine the chemical and optical purity of α- amino acids and esters, it is expected to be quite useful for their chiral separation using Chiralpak IE.

영어초록

Liquid chromatographic enantiomer separation of α-amino acids and their methyl and ethyl esters as fluorenylmethoxycarbonyl (FMOC) derivatives was performed using a recently developed chiral column (Chiralpak IE) based on polysaccharide derivative under simultaneous UV detection and fluorescence detection. The degree of enantiomer separation of α-amino acid esters as FMOC derivatives is generally higher than that of the corresponding α-amino acids. Especially, α-amino acid methyl esters showed the greatest enantioseparation.
As this method developed in this study can be applied to determine the chemical and optical purity of α- amino acids and esters, it is expected to be quite useful for their chiral separation using Chiralpak IE.

참고자료

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