4주간의 후지체공 자극이 흰쥐의 골밀도 및 Ca대사에 미치는 영향 (Effects of Stimulation of Hindlimb Suspension on Bone Mineral Density and Ca Metabolism for 4 Weeks in Rats.)

서지정보

· 발행기관 : 한국운동영양학회
· 수록지 정보 : Physical Activity and Nutrition / 12권 / 3호 / 149 ~ 155페이지
· 저자명 : 김창선, 이신언

초록

This study was designed to evaluate the effects of stimulation of hindlimb suspension on bone mineral density and the Ca metabolism for 4 weeks in rats. Eighteen 7-week-old female Sprague-Dawley (SD) rats completed hindlimb suspension for 4 weeks. After a week of stabilization, rats were randomly divided into three groups: baseline controls group (n=6, BC), 4-week vivarium control group (n=6, Con) and 4-week hindlimb suspension group (n=6, Sus). All rats were fed standard lab chow and to have same-volume-feeding in all groups. After 4 weeks suspension, rats were sacrificed and femurs were removed for analysis of bone mineral density (BMD) by dual energy X-ray absorptiometry (DEXA, Lunar DPXL, USA). The body weight, gain of body weight per a week and food intake of the Con and the Sus group were significantly increased compared to the BC group (p<.05, p<.01, p<.001, respectively), but there were no significantly change between the Con and the Sus group. The femur mass of the Con group was increased approximately in 10.5% as compared to the BC group (p<.05) while that of the Sus group showed no change. Similarly, f-BMD and f-BMC of the Con and the Sus group were increased as compared with those of the BC group (p<.05, p<.001, respectively), but those of the Sus group showed approximately 13.9 and 17.3 % lower than those of the Con group (p<.001, respectively). On the other hand, the serum calcium (Ca) concentration, free Ca index and 24h urinary Ca excretions between the Con and the Sus group showed similar levels. However, urinary phosphate (P) excretion of the Sus group dynamically decreased approximately 48.1% lower than that of the Con group (p<.01) while serum P concentration and ratio of P/Ca was increased (p<.05, respectively). These results provide evidence for the consideration of bone loss when exposure to the inactivity and weightless and this osteopenia might be caused by disturbed P homeostasis.

영어초록

This study was designed to evaluate the effects of stimulation of hindlimb suspension on bone mineral density and the Ca metabolism for 4 weeks in rats. Eighteen 7-week-old female Sprague-Dawley (SD) rats completed hindlimb suspension for 4 weeks. After a week of stabilization, rats were randomly divided into three groups: baseline controls group (n=6, BC), 4-week vivarium control group (n=6, Con) and 4-week hindlimb suspension group (n=6, Sus). All rats were fed standard lab chow and to have same-volume-feeding in all groups. After 4 weeks suspension, rats were sacrificed and femurs were removed for analysis of bone mineral density (BMD) by dual energy X-ray absorptiometry (DEXA, Lunar DPXL, USA). The body weight, gain of body weight per a week and food intake of the Con and the Sus group were significantly increased compared to the BC group (p<.05, p<.01, p<.001, respectively), but there were no significantly change between the Con and the Sus group. The femur mass of the Con group was increased approximately in 10.5% as compared to the BC group (p<.05) while that of the Sus group showed no change. Similarly, f-BMD and f-BMC of the Con and the Sus group were increased as compared with those of the BC group (p<.05, p<.001, respectively), but those of the Sus group showed approximately 13.9 and 17.3 % lower than those of the Con group (p<.001, respectively). On the other hand, the serum calcium (Ca) concentration, free Ca index and 24h urinary Ca excretions between the Con and the Sus group showed similar levels. However, urinary phosphate (P) excretion of the Sus group dynamically decreased approximately 48.1% lower than that of the Con group (p<.01) while serum P concentration and ratio of P/Ca was increased (p<.05, respectively). These results provide evidence for the consideration of bone loss when exposure to the inactivity and weightless and this osteopenia might be caused by disturbed P homeostasis.

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