티타늄 Particle농도에 따른 치주인대세포내 세포독성 및 OPG, RANKL의 발현에 관한 연구

서지정보

· 발행기관 : 대한구강악안면병리학회
· 수록지 정보 : 대한구강악안면병리학회지 / 35권 / 2호
· 저자명 : 조형준, 나유진, 정용석, 홍지숙, 김윤지, 이종헌, 천재식, 박양호

목차

I. 서론
II. 재료 및 방법
1. 연구방법
2. 통계처리
III. 결과
1. 치주인대세포주의 생존력 분석
2. Reverse Transcriptase-Polymerase ChainReaction(RT-PCR)분석법에 의한 RANKL, OPG발현
3. Western blot analysis를 이용한 OPG, RANKLProtein의 발현
IV. 고찰
V. 결론
VI. 참고문헌

영어초록

The purpose of this study was to investigate the effect of cytokine-induced osteoclastogenesis on tooth movement related to orthodontic force. We evaluated the cytotoxicity as well as the expression of OPG and RANKL, which influence the homeostasis of bone metabolism. Titanium particles were applied to human periodontal ligament cells and subcultured fourth generation cells. The ALP assay and the MTT assay were used to assess changes in cytotoxicity. After 48 hours, cytotoxicity increased proportionally with the concentration of titanium. With 20 mg, the cytotoxicity was the lowest. R T-PCR was u sed for assessing m R NA l evels of O PG a nd R ANKL; after 96 hours, t he m R NAs of O PG a nd R ANKL increased steeply. A western blot analysis showed that with 20 mg of titanium, the protein expression of OPG increased linearly with time, especially a fter 96 hours, while t he p rotein e xpression o f RANKL d id n ot s how significant changes with titanium processing. Given the increase in OPG expression after the initial cytotoxicity, changes in cytotoxicity with titanium may be attributable to the antagonistic effect of OPG on cytotoxicity

참고자료

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